![]() ![]() This study aims to investigate liver cell responses to topographical features on electrospun fibres. In the present study, we show that hepatocyte-like cells differentiated from USSCs on the PCL nanofiber scaffold can be candidate for tissue engineering and cell therapy of hepatic tissues. Electrospinning is a well-known method to fabricate a nanofibre scaffold which mimics the natural extracellular matrix that can support cell growth. RT-PCR analysis of endoderm and hepatic-specific gene expression, immunohistochemical detection of cytokeratin 18 (CK-18), α-fetoprotein, albumin, glycogen storage and indocyanine green uptake confirmed the differentiation of USSCs into endoderm and hepatocyte-like cells. USSCs were seeded onto PCL nanofiber scaffolds, and were induced to differentiate into hepatogenic lineages by culturing with differentiation factors for 6 weeks. The electrospun PCL nanofiber porous scaffold was constructed of uniform, randomly oriented nanofibers. USSCs, self-renewing pluripotent cells, were isolated from human cord blood. In this study, we tested the ability of poly(ε-caprolactone) (PCL) nanofiber scaffold to support and maintain hepatic differentiation of human cord blood-derived unrestricted somatic stem cells (USSCs) in vitro. doi: 10.1016/j.ydbio.2016.06.036.Tissue engineering of implantable cellular constructs is an emerging cellular therapy for hepatic disease. EB formation and hepatic differentiation. Reverse Engineering Liver Buds Through Self-Driven Condensation And Organization Towards Medical Application. Neves1,2 Electrospinning has attracted tremendous interest in the research community as a simple and versatile technique to produce synthetic polymeric ultrafine fibres with diameters ranging from a few micrometres to tens of nanometres. Implementation of the Lancet Standing Commission on Liver Disease in the UK. Electrospinning: processing technique for tissue engineering scaffolding A. The Lancet Commissions Addressing liver disease in the UK: a blueprint for attaining excellence in health care and reducing premature mortality from lifestyle issues of excess consumption of alcohol, obesity, and viral hepatitis. The burden of liver disease in Europe: A review of available epidemiological data. 2017 (2017).īlachier M, Leleu H, Peck-Radosavljevic M, Valla D-C, Roudot-Thoraval F. Many approaches have been used such as direct cellular injection onto present vascular beds, micro-carrier attachment and scaffold implants seeded with cells. Blended protein:polymer scaffolds provide a viable, translatable niche for hepatocytes and offers a solution to current obstacles in disease modelling and liver tissue engineering. Hepatic tissue engineering is focused on creating a whole, implantable and functional liver. We first describe three major methods for nanofibrous scaffold fabrication: molecular self-assembly, phase separation, and electrospinning. Each scaffold maintained hepatocyte growth, albumin production and influenced expression of key hepatic genes, with the decellularized ECM scaffolds exerting an influence which is not recapitulated by individual ECM components. Mechanical testing demonstrated significant increases in the Young's Modulus of the decellularized ECM scaffold providing significantly stiffer environments for hepatocytes. Immunohistochemistry confirmed retention of proteins in the scaffolds. The resulting scaffolds were validated using THLE-3 hepatocytes. We combined decellularized human liver tissue with electrospun polymers to produce a niche for hepatocytes and compared the human liver ECM to its individual components Collagen I, Laminin-521 and Fibronectin. PLGA knitted mesh-reinforced colla- gen-chitosan scaffolds for the increase of scaffold function pro- moted faster cell infiltration and ECM formation. Enhancing microenvironments using bioactive molecules allows researchers to create more appropriate niches for hepatocytes. Polymers and decellularized tissue scaffolds each provide some of the necessary biological cues for hepatocytes, however, neither alone has proved sufficient. Researchers have developed a more accurate model of this scarred cardiac tissue that could allow for quicker drug testing. One of the challenges for tissue engineers is the extracellular matrix (ECM) a finely controlled in vivo niche which supports hepatocytes. But recent research published in ACS Biomaterials Science & Engineering could open up new pathways toward potential therapies by focusing instead on cardiac fibrosis a condition that often precedes heart failure. Tissue engineering of a transplantable liver could provide an alternative to donor livers for transplant, solving the problem of escalating donor shortages. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |